Hotspot

trnE-UUC-trnT-GGU

LSC · 34354-35355 · 1002 bp

Back to Castanopsis

Pi 0.00131

MarkerSeek score 57.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1002
variable_sites: 5
pi: 0.00131
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	ACTTCCTTGCGTAGTACCCT	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1131-1154 bp	1.0	83.3
trnE-UUC-trnT-GGU_p2	ACTTCCTTGCGTAGTACCCT	CCATGGCGTTACTCTACCAC	50.0	55.0	58.1	58.1	1110-1133 bp	1.0	83.0
trnE-UUC-trnT-GGU_p3	ACTTCCTTGCGTAGTACCCT	TGGCGTTACTCTACCACTGA	50.0	50.0	58.1	58.1	1107-1130 bp	1.0	82.8
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	1088-1111 bp	1.0	82.6
trnE-UUC-trnT-GGU_p5	ACTTCCTTGCGTAGTACCCT	CCGATGACTTACGCCTTACC	50.0	55.0	58.1	58.2	1128-1151 bp	1.0	82.2