Hotspot

rpoB-trnF-GAA

LSC · 28895-30118 · 1224 bp

Back to Castanopsis

Pi 0.00708

MarkerSeek score 66.70

Primer available yes

Label rpoB-trnF-GAA

Feature Metadata

label_name: rpoB-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1224
variable_sites: 34
pi: 0.00708
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnF-GAA_p1	CCTCATTTCTACCCCCAAGC	TTAGGCGACACCCAGATTTG	55.0	50.0	57.9	57.9	1240-1466 bp	1.0	84.7
rpoB-trnF-GAA_p2	CCTCATTTCTACCCCCAAGC	TTGTTGATTAGGCGACACCC	55.0	50.0	57.9	58.2	1247-1473 bp	1.0	84.1
rpoB-trnF-GAA_p3	CCTCATTTCTACCCCCAAGC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1211-1437 bp	1.0	82.7
rpoB-trnF-GAA_p4	TCCCTCATTTCTACCCCCAA	TTAGGCGACACCCAGATTTG	50.0	50.0	57.6	57.9	1242-1468 bp	1.0	82.2
rpoB-trnF-GAA_p5	TTCCCTCATTTCTACCCCCA	TTAGGCGACACCCAGATTTG	50.0	50.0	57.6	57.9	1243-1469 bp	1.0	82.2