Hotspot

trnS-GCU-trnG-GCC

LSC · 9327-10268 · 942 bp

Back to Castanopsis

Pi 0.00344
MarkerSeek score 61.10
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
942
variable_sites
14
pi
0.00344
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA CGAAGGACCCCTTAACCATT 50.0 50.0 58.0 57.2 1045-1096 bp 1.0 76.0
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC CGAAGGACCCCTTAACCATT 50.0 50.0 58.0 57.2 1044-1095 bp 1.0 76.0
trnS-GCU-trnG-GCC_p3 TAGTCCACTCAGCCATCTCC CGAAGGACCCCTTAACCATT 55.0 50.0 58.2 57.2 1032-1083 bp 1.0 74.4
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA CGAAGGACCCCTTAACCATTT 50.0 47.6 58.0 57.9 1045-1096 bp 1.0 73.8
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC CGAAGGACCCCTTAACCATTT 50.0 47.6 58.0 57.9 1044-1095 bp 1.0 73.8