Hotspot

psbK-psbI

LSC · 8569-9005 · 437 bp

Back to Castanopsis

Pi 0.0164
MarkerSeek score 62.50
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
437
variable_sites
34
pi
0.0164
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG CTTCACTCTTCACGTCCAGG 50.0 55.0 57.9 57.9 537-557 bp 1.0 85.1
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG TCACTCTTCACGTCCAGGAT 50.0 50.0 57.9 58.1 535-555 bp 1.0 85.1
psbK-psbI_p3 CTTTTGTTTGGCAAGCTGCT CTTCACTCTTCACGTCCAGG 45.0 55.0 58.1 57.9 542-562 bp 1.0 85.0
psbK-psbI_p4 CTTTTGTTTGGCAAGCTGCT TCACTCTTCACGTCCAGGAT 45.0 50.0 58.1 58.1 540-560 bp 1.0 85.0
psbK-psbI_p5 CTTTTGTTTGGCAAGCTGCT TTCACTCTTCACGTCCAGGA 45.0 50.0 58.1 58.3 541-561 bp 1.0 83.2