Hotspot

trnK-UUU-rps16

LSC · 4433-5267 · 835 bp

Back to Castanopsis

Pi 0.0104

MarkerSeek score 61.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 26
pi: 0.0104
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TATTTCCTTGAAAGGGGCGC	50.0	50.0	58.2	58.5	963-979 bp	1.0	80.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TATTTCCTTGAAAGGGGCGC	50.0	50.0	58.2	58.5	962-978 bp	1.0	80.6
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	CGAAGAAGGCGGGAGTTTTT	50.0	50.0	58.2	58.8	913-929 bp	1.0	78.9
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	TATTTCGAAGAAGGCGGGAG	50.0	50.0	58.2	57.4	919-935 bp	0.909091	76.5
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TATTTCGAAGAAGGCGGGAG	50.0	50.0	58.2	57.4	918-934 bp	0.909091	76.5