Hotspot

rps16

LSC · 5463-6643 · 1181 bp

Back to Castanea

Pi 0.00682

MarkerSeek score 51.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1181
variable_sites: 12
pi: 0.00682
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCCCCTCTTTTGTTCTATTCA	ACAGCAACCTACTAGGAAATGT	42.9	40.9	55.2	57.1	1198-1225 bp	1.0	42.6
rps16_p2	CCCCCTCTTTTGTTCTATTCA	ACAGCAACCTACTAGGAAATGTT	42.9	39.1	55.2	57.8	1198-1225 bp	1.0	42.6
rps16_p3	CCCCCTCTTTTGTTCTATTCA	CTGTTTACAGCAACCTACTAGGA	42.9	43.5	55.2	57.6	1204-1231 bp	1.0	42.6
rps16_p4	CCCCCTCTTTTGTTCTATTCA	TGTTTACAGCAACCTACTAGGA	42.9	40.9	55.2	56.5	1203-1230 bp	1.0	42.6
rps16_p5	CCCCCTCTTTTGTTCTATTCA	CAGCAACCTACTAGGAAATGT	42.9	42.9	55.2	55.3	1197-1224 bp	1.0	42.6