Hotspot

ndhF

SSC · 116088-118367 · 2280 bp

Back to Castanea

Pi 0.00466

MarkerSeek score 55.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2280
variable_sites: 20
pi: 0.00466
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCACTTTAAAGGGACACGCT	AAGAACTGGCGAATCCGAAA	50.0	45.0	58.1	57.8	2424-2450 bp	1.0	78.5
ndhF_p2	TCCACTTTAAAGGGACACGC	AAGAACTGGCGAATCCGAAA	50.0	45.0	58.1	57.8	2425-2451 bp	1.0	78.5
ndhF_p3	CCACTTTAAAGGGACACGCT	TGGCGAATCCGAAACAGTTA	50.0	45.0	58.1	57.5	2418-2444 bp	1.0	76.3
ndhF_p4	TCCACTTTAAAGGGACACGC	TGGCGAATCCGAAACAGTTA	50.0	45.0	58.1	57.5	2419-2445 bp	1.0	76.2
ndhF_p5	CCACTTTAAAGGGACACGCT	AGAACTGGCGAATCCGAAAC	50.0	50.0	58.1	58.6	2423-2449 bp	1.0	75.5