Hotspot

ndhA

SSC · 126325-128467 · 2143 bp

Back to Castanea

Pi 0.00426

MarkerSeek score 61.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2143
variable_sites: 20
pi: 0.00426
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTTGTTTGAGGCAAGCCAT	TGGGAGAAGTTGATCGTTGA	45.0	45.0	58.0	56.5	2194-2207 bp	1.0	69.4
ndhA_p2	TCTTGTTTGAGGCAAGCCAT	TGGGAGAAGTTGATCGTTGAA	45.0	42.9	58.0	57.2	2194-2207 bp	1.0	67.4
ndhA_p3	TCTCTTGTTTGAGGCAAGCC	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.4	56.5	2196-2209 bp	1.0	66.3
ndhA_p4	TCTTGTTTGAGGCAAGCCAT	ATGGGAGAAGTTGATCGTTGA	45.0	42.9	58.0	57.0	2195-2208 bp	1.0	65.6
ndhA_p5	TCTCTTGTTTGAGGCAAGCC	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	58.4	57.2	2196-2209 bp	1.0	64.3