Hotspot

clpP

LSC · 75957-78038 · 2082 bp

Back to Castanea

Pi 0.00375

MarkerSeek score 48.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2082
variable_sites: 16
pi: 0.00375
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCGTATTTGTATTTGCGCGA	CGTTGTTACGCTTCCACATC	45.0	50.0	57.9	57.5	2173-2184 bp	1.0	76.0
clpP_p2	GTATTTGCGCGAAATCCCTG	CGTTGTTACGCTTCCACATC	50.0	50.0	57.9	57.5	2165-2176 bp	1.0	75.8
clpP_p3	TGGCGTATTTGTATTTGCGC	CGTTGTTACGCTTCCACATC	45.0	50.0	57.8	57.5	2175-2186 bp	1.0	75.2
clpP_p4	TGCGCGAAATCCCTGTTATT	CGTTGTTACGCTTCCACATC	45.0	50.0	58.3	57.5	2160-2171 bp	1.0	74.6
clpP_p5	TTGCGCGAAATCCCTGTTAT	CGTTGTTACGCTTCCACATC	45.0	50.0	58.3	57.5	2161-2172 bp	1.0	74.6