Hotspot

rpl32-trnL-UAG

SSC · 119479-120654 · 1176 bp

Back to Castanea

Pi 0.00426

MarkerSeek score 60.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1176
variable_sites: 11
pi: 0.00426
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGGAGGGGTATTGGGTAG	AGCGTGTCTACCGATTTCAC	55.0	50.0	57.8	58.0	1300-1316 bp	1.0	80.0
rpl32-trnL-UAG_p2	AAGGGAGGGGTATTGGGTAG	CAGCGTGTCTACCGATTTCA	55.0	50.0	57.8	58.0	1301-1317 bp	1.0	80.0
rpl32-trnL-UAG_p3	AAAAGGGAGGGGTATTGGGT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1302-1318 bp	1.0	80.0
rpl32-trnL-UAG_p4	AAAAGGGAGGGGTATTGGGT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1303-1319 bp	1.0	79.9
rpl32-trnL-UAG_p5	AAGGGAGGGGTATTGGGTAG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	57.8	57.9	1310-1326 bp	1.0	79.1