Hotspot

rpl14-rpl16

LSC · 87082-87232 · 151 bp

Back to Castanea

Pi 0.00608

MarkerSeek score 58.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 2
pi: 0.00608
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTGTTATCCGCTACATT	AATAGCGGCATCCAAAATGC	50.0	45.0	58.1	57.5	238-247 bp	1.0	78.4
rpl14-rpl16_p2	CCGCTGTTATCCGCTACATT	TGAAATGGGTGGAGTAGCAG	50.0	50.0	58.1	57.2	283-292 bp	1.0	75.7
rpl14-rpl16_p3	ACACATTAATTGTCGGGCCC	AATAGCGGCATCCAAAATGC	50.0	45.0	58.5	57.5	257-266 bp	1.0	75.0
rpl14-rpl16_p4	CCGCTGTTATCCGCTACATT	CCAAAATGCCTATACGAACGC	50.0	47.6	58.1	58.0	227-236 bp	1.0	74.6
rpl14-rpl16_p5	CCGCTGTTATCCGCTACATT	TGGGTGGAGTAGCAGAAAATG	50.0	47.6	58.1	57.9	278-287 bp	1.0	73.5