Hotspot

psbE-petL

LSC · 70325-71655 · 1331 bp

Back to Castanea

Pi 0.00887

MarkerSeek score 66.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1331
variable_sites: 23
pi: 0.00887
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGTTCAACCCG	50.0	47.6	57.8	58.0	1440-1469 bp	1.0	72.4
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGTTCAACCCGA	50.0	47.6	57.8	58.0	1439-1468 bp	1.0	72.4
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGTCGTATCTTGTTCAACCCG	45.0	47.6	58.2	58.0	1526-1555 bp	1.0	72.2
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGTTCAACCCGA	45.0	47.6	58.2	58.0	1525-1554 bp	1.0	72.2
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGTCGTATCTTGTTCAACCCG	50.0	47.6	58.2	58.0	1531-1560 bp	1.0	72.1