Hotspot

petA-psbJ

LSC · 68517-69542 · 1026 bp

Back to Castanea

Pi 0.0039

MarkerSeek score 62.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1026
variable_sites: 8
pi: 0.0039
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1164-1171 bp	1.0	78.0
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1161-1168 bp	1.0	76.9
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1159-1166 bp	1.0	75.5
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	AGGCACTGTAACGGGTATTC	50.0	50.0	58.0	57.3	1223-1230 bp	1.0	75.4
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	GGCACTGTAACGGGTATTCT	50.0	50.0	58.0	57.3	1222-1229 bp	1.0	75.4