Hotspot

accD-psaI

LSC · 64202-64898 · 697 bp

Back to Castanea

Pi 0.00631

MarkerSeek score 48.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 8
pi: 0.00631
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACCAATCGGTTTTTGCCTCT	AAAGAAGCCATCGCAATTGC	45.0	45.0	58.0	58.0	1218-1257 bp	1.0	83.6
accD-psaI_p2	GCTACCAATCGGTTTTTGCC	AAAGAAGCCATCGCAATTGC	50.0	45.0	58.0	58.0	1221-1260 bp	1.0	83.6
accD-psaI_p3	GGTAGTATGGGCTCCGTAGT	AAAGAAGCCATCGCAATTGC	55.0	45.0	58.0	58.0	1272-1311 bp	1.0	83.4
accD-psaI_p4	ACCAATCGGTTTTTGCCTCT	CCCCGATCCCGATTTGATTT	45.0	50.0	58.0	58.0	1465-1508 bp	1.0	83.0
accD-psaI_p5	GGTAGTATGGGCTCCGTAGT	CCCCGATCCCGATTTGATTT	55.0	50.0	58.0	58.0	1519-1562 bp	1.0	82.9