Hotspot

rbcL-accD

LSC · 61992-62590 · 599 bp

Back to Castanea

Pi 0.0267

MarkerSeek score 75.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 29
pi: 0.0267
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTGCTAAATGGAGTCC	AACATCGAGTTGAACCACCC	55.0	50.0	58.0	58.1	769 bp	1.0	81.8
rbcL-accD_p2	GAGGCTGCTAAATGGAGTCC	CATCGAGTTGAACCACCCTT	55.0	50.0	58.0	57.8	767 bp	1.0	81.2
rbcL-accD_p3	AATGGAGTCCTGAGCTAGCT	AACATCGAGTTGAACCACCC	50.0	50.0	57.8	58.1	759 bp	1.0	80.8
rbcL-accD_p4	AATGGAGTCCTGAGCTAGCT	CATCGAGTTGAACCACCCTT	50.0	50.0	57.8	57.8	757 bp	1.0	80.2
rbcL-accD_p5	TTCGTGAGGCTGCTAAATGG	AACATCGAGTTGAACCACCC	50.0	50.0	58.3	58.1	774 bp	1.0	80.0