Hotspot

psbC-trnS-UGA

LSC · 39700-39966 · 267 bp

Back to Castanea

Pi 0.0184

MarkerSeek score 61.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 9
pi: 0.0184
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TTTATGGCATGCAGGAAGGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.2	58.1	440-451 bp	1.0	80.3
psbC-trnS-UGA_p2	TTTATGGCATGCAGGAAGGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	386-397 bp	1.0	79.4
psbC-trnS-UGA_p3	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	413-424 bp	1.0	79.2
psbC-trnS-UGA_p4	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	412-423 bp	1.0	78.7
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.4	58.2	359-370 bp	1.0	78.3