Hotspot

trnE-UUC-trnT-GGU

LSC · 34529-35538 · 1010 bp

Back to Castanea

Pi 0.00515

MarkerSeek score 64.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 12
pi: 0.00515
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	ACTTCCTTGCGTAGTACCCT	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1150-1157 bp	1.0	81.2
trnE-UUC-trnT-GGU_p2	ACTTCCTTGCGTAGTACCCT	CCATGGCGTTACTCTACCAC	50.0	55.0	58.1	58.1	1129-1136 bp	1.0	80.8
trnE-UUC-trnT-GGU_p3	ACTTCCTTGCGTAGTACCCT	TGGCGTTACTCTACCACTGA	50.0	50.0	58.1	58.1	1126-1133 bp	1.0	80.6
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	1107-1114 bp	1.0	80.5
trnE-UUC-trnT-GGU_p5	ACTTCCTTGCGTAGTACCCT	CCGATGACTTACGCCTTACC	50.0	55.0	58.1	58.2	1147-1154 bp	1.0	80.1