Hotspot

trnK-UUU-rps16

LSC · 4401-5462 · 1062 bp

Back to Castanea

Pi 0.013
MarkerSeek score 61.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1062
variable_sites
27
pi
0.013
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAGGCGGGAGTTTTTATGGA 50.0 45.0 58.2 57.1 1114-1162 bp 1.0 76.2
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AGGCGGGAGTTTTTATGGAA 50.0 45.0 58.2 57.1 1113-1161 bp 1.0 76.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAGGCGGGAGTTTTTATGGA 50.0 45.0 58.2 57.1 1113-1161 bp 1.0 76.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGGCGGGAGTTTTTATGGAA 50.0 45.0 58.2 57.1 1112-1160 bp 1.0 76.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGCGGGAGTTTTTATGGAAC 50.0 50.0 58.2 57.1 1111-1159 bp 1.0 75.9