Hotspot

trnH-GUG-psbA

LSC · 77-508 · 432 bp

Back to Castanea

Pi 0.0231

MarkerSeek score 69.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 19
pi: 0.0231
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CGTGCTAACCTTGGTATGGA	55.0	50.0	58.1	57.3	537-557 bp	1.0	79.5
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TCGTGCTAACCTTGGTATGG	55.0	50.0	58.1	57.3	538-558 bp	1.0	79.5
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.9	57.3	551-571 bp	1.0	79.3
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	TCGTGCTAACCTTGGTATGG	50.0	50.0	57.9	57.3	552-572 bp	1.0	79.3
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.9	57.3	550-570 bp	1.0	79.3