Hotspot

atpE

Genome · 49313-49717 · 405 bp

Back to Cassytha

Pi 0.00494
MarkerSeek score 44.00
Primer available yes
Label atpE

Feature Metadata

label_name
atpE
feature_type
gene
region
Genome
length_bp
405
variable_sites
2
pi
0.00494
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE_p1 GGTACCTAAATCGGCACCTG CTGCTCCTGCTACGACATTT 55.0 50.0 58.1 58.0 1513-1515 bp 1.0 81.2
atpE_p2 TAAATCGGCACCTGAATCGG CTGCTCCTGCTACGACATTT 50.0 50.0 58.1 58.0 1507-1509 bp 1.0 81.2
atpE_p3 GGTACCTAAATCGGCACCTG GCAGTTTATGTACCTGCGGA 55.0 50.0 58.1 58.0 1547-1549 bp 1.0 81.1
atpE_p4 GGTACCTAAATCGGCACCTG TTGGTTTGACTGCCCTAACC 55.0 50.0 58.1 58.0 1777-1779 bp 1.0 81.0
atpE_p5 TAAATCGGCACCTGAATCGG TTGGTTTGACTGCCCTAACC 50.0 50.0 58.1 58.0 1771-1773 bp 1.0 80.9