Hotspot

trnA-UGC-trnI-GAU

Genome · 107372-107435 · 64 bp

Back to Cassytha

Pi 0.0312
MarkerSeek score 67.00
Primer available yes
Label trnA-UGC-trnI-GAU

Feature Metadata

label_name
trnA-UGC-trnI-GAU
feature_type
igs
region
Genome
length_bp
64
variable_sites
2
pi
0.0312
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC-trnI-GAU_p1 TACCGCCTGCACAATTAGAC TGATTTACTTCACGGGCGAG 50.0 50.0 58.0 58.0 197 bp 1.0 80.6
trnA-UGC-trnI-GAU_p2 TACCGCCTGCACAATTAGAC ATTGGTTGGATCGTAGGTGC 50.0 50.0 58.0 58.0 219 bp 1.0 80.3
trnA-UGC-trnI-GAU_p3 TACCGCCTGCACAATTAGAC AGGTCTCTGGTTCAAGTCCA 50.0 50.0 58.0 57.9 179 bp 1.0 79.9
trnA-UGC-trnI-GAU_p4 CAACGACCCAATTGCAAGAG TGATTTACTTCACGGGCGAG 50.0 50.0 57.7 58.0 161 bp 1.0 78.3
trnA-UGC-trnI-GAU_p5 TACCGCCTGCACAATTAGAC CGAGGTCTCTGGTTCAAGTC 50.0 55.0 58.0 57.6 181 bp 1.0 77.9