Hotspot

rpl32-trnL-UAG

Genome · 90358-91349 · 992 bp

Back to Cassytha

Pi 0.0562
MarkerSeek score 89.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
992
variable_sites
55
pi
0.0562
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
89.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CCATTTCTACCGGGCATTCA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1079-1086 bp 1.0 84.6
rpl32-trnL-UAG_p2 AAAGGGATATCAAGCCGCTG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1123-1130 bp 1.0 84.5
rpl32-trnL-UAG_p3 AAAGGGATATCAAGCCGCTG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1124-1131 bp 1.0 84.5
rpl32-trnL-UAG_p4 CCATTTCTACCGGGCATTCA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1080-1087 bp 1.0 84.5
rpl32-trnL-UAG_p5 AAAGGGATATCAAGCCGCTG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1133-1140 bp 1.0 83.6