Hotspot

psbT-psbN

Genome · 70986-71043 · 58 bp

Back to Cassytha

Pi 0.0517

MarkerSeek score 70.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: Genome
length_bp: 58
variable_sites: 3
pi: 0.0517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTAAGG	TCGCCATCTCCATATCTGGT	50.0	50.0	57.9	58.0	214 bp	1.0	80.3
psbT-psbN_p2	TTTTCGAGAACCGCCTAAGG	GGGTACGCCTTATATACCGC	50.0	55.0	57.9	57.7	176 bp	1.0	78.4
psbT-psbN_p3	TTTTCGAGAACCGCCTAAGG	GATCCATTCGAGGAACACGG	50.0	55.0	57.9	58.4	128 bp	1.0	77.7
psbT-psbN_p4	TTTTCGAGAACCGCCTAAGG	GTCGCCATCTCCATATCTGG	50.0	55.0	57.9	57.6	215 bp	1.0	77.4
psbT-psbN_p5	TTTTCGAGAACCGCCTAAGG	GGCAACCCTCTCAACAACTA	50.0	50.0	57.9	57.4	151 bp	1.0	76.4