Hotspot

psbE-petL

Genome · 61516-62789 · 1274 bp

Back to Cassytha

Pi 0.0165

MarkerSeek score 68.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1274
variable_sites: 21
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1421-1425 bp	1.0	77.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1419-1423 bp	1.0	77.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1430-1434 bp	1.0	76.2
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.2	58.5	1428-1432 bp	1.0	76.2
psbE-petL_p5	TGCTGACGAATAACCAACCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1435-1439 bp	1.0	76.1