Hotspot

petA-psbJ

Genome · 59754-60741 · 988 bp

Back to Cassytha

Pi 0.0255

MarkerSeek score 74.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 988
variable_sites: 25
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	ATTATAGGCACTGCAACGGG	45.0	50.0	58.2	58.0	1190 bp	1.0	80.7
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	TATAGGCACTGCAACGGGTA	45.0	50.0	58.2	58.5	1188 bp	1.0	76.8
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	TGCAACGGGTATTCTTGTGA	45.0	45.0	58.2	57.4	1179 bp	1.0	76.5
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	ACTGCAACGGGTATTCTTGT	45.0	45.0	58.2	57.4	1181 bp	1.0	76.4
petA-psbJ_p5	CGTGTCCAAGGTTTGTTGTT	ATTATAGGCACTGCAACGGG	45.0	50.0	57.1	58.0	1189 bp	1.0	74.6