Hotspot

trnM-CAU-atpE

Genome · 49129-49312 · 184 bp

Back to Cassytha

Pi 0.106

MarkerSeek score 76.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: Genome
length_bp: 184
variable_sites: 19
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TGGTTCAAATCCAATAGTAGGT	GAGTGGAGGCTGTCAATGTT	36.4	50.0	54.8	57.8	238-239 bp	1.0	47.7
trnM-CAU-atpE_p2	TGGTTCAAATCCAATAGTAGGT	TAGACGAGCTAGGACACGAG	36.4	55.0	54.8	58.1	255-256 bp	1.0	47.2
trnM-CAU-atpE_p3	TGGTTCAAATCCAATAGTAGGT	GCTGAAGGCAAGAGACAAGT	36.4	50.0	54.8	58.1	296-297 bp	1.0	46.2
trnM-CAU-atpE_p4	TGGTTCAAATCCAATAGTAGGT	GAGCTGAAGGCAAGAGACAA	36.4	50.0	54.8	57.8	298-299 bp	1.0	46.2
trnM-CAU-atpE_p5	TGGTTCAAATCCAATAGTAGGT	AGCTGAAGGCAAGAGACAAG	36.4	50.0	54.8	57.8	297-298 bp	1.0	46.2