Hotspot

rps2-rpoC2

Genome · 15819-16049 · 231 bp

Back to Cassytha

Pi 0.0354

MarkerSeek score 64.60

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: Genome
length_bp: 231
variable_sites: 8
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GCTTCCATCATCTCTGCCAA	TGGTTCTTGCATCCCAAACA	50.0	45.0	58.0	57.9	319-324 bp	1.0	82.2
rps2-rpoC2_p2	TTGCAGAGATGTAAGGTGCC	TGGTTCTTGCATCCCAAACA	50.0	45.0	57.9	57.9	384-389 bp	1.0	81.1
rps2-rpoC2_p3	GCAGAGATGTAAGGTGCCAT	TGGTTCTTGCATCCCAAACA	50.0	45.0	57.7	57.9	382-387 bp	1.0	79.4
rps2-rpoC2_p4	CCTGCTTCCATCATCTCTGC	TGGTTCTTGCATCCCAAACA	55.0	45.0	58.4	57.9	322-327 bp	1.0	79.3
rps2-rpoC2_p5	CTGCTTCCATCATCTCTGCC	TGGTTCTTGCATCCCAAACA	55.0	45.0	58.4	57.9	321-326 bp	1.0	79.3