Hotspot

trnQ-UUG-psbK

Genome · 6907-7246 · 340 bp

Back to Cassytha

Pi 0.0297
MarkerSeek score 69.50
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
Genome
length_bp
340
variable_sites
10
pi
0.0297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 CAAAACCCGTAGCCTTACCA TTTGGCGAAGAAAAAGCGAC 50.0 45.0 57.8 57.9 444-459 bp 1.0 79.9
trnQ-UUG-psbK_p2 AAAACCCGTAGCCTTACCAC TTTGGCGAAGAAAAAGCGAC 50.0 45.0 57.8 57.9 443-458 bp 1.0 79.9
trnQ-UUG-psbK_p3 ACCAAAACCCGTAGCCTTAC TTTGGCGAAGAAAAAGCGAC 50.0 45.0 57.8 57.9 446-461 bp 1.0 79.9
trnQ-UUG-psbK_p4 GAAGGATTCGAACCTCCGAG TTTGGCGAAGAAAAAGCGAC 55.0 45.0 57.8 57.9 473-488 bp 1.0 79.6
trnQ-UUG-psbK_p5 CAAAACCCGTAGCCTTACCA GGGCAATTTGGCGAAGAAAA 50.0 45.0 57.8 57.8 450-465 bp 1.0 79.5