Hotspot

accD-psaI

LSC · 63392-64010 · 619 bp

Back to Cassia

Pi 0.0441
MarkerSeek score 83.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
619
variable_sites
25
pi
0.0441
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TTGATTCAATCGTACCGCGT TGGAGGGTAAGTTGCTGAGA 45.0 50.0 58.0 58.0 728-736 bp 1.0 86.5
accD-psaI_p2 TTGATTCAATCGTACCGCGT CCATTGCAATTGCCGGAAAT 45.0 45.0 58.0 58.0 773-781 bp 1.0 86.4
accD-psaI_p3 TTGATTCAATCGTACCGCGT AAGGCACAAAAATGGAGGGT 45.0 45.0 58.0 57.9 740-748 bp 1.0 85.9
accD-psaI_p4 TTGATTCAATCGTACCGCGT AAGAAGCCATTGCAATTGCC 45.0 45.0 58.0 57.9 779-787 bp 1.0 85.7
accD-psaI_p5 TCAATCGTACCGCGTAATCC TGGAGGGTAAGTTGCTGAGA 50.0 50.0 57.9 58.0 723-731 bp 1.0 85.6