Hotspot

psaA-ycf3

LSC · 46214-46999 · 786 bp

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Pi 0.0171
MarkerSeek score 71.60
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
786
variable_sites
13
pi
0.0171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CCCATTCCTCGAAAGACGTT AGCCCTTACCCCTGGTAATT 50.0 50.0 57.9 58.0 926-932 bp 1.0 82.5
psaA-ycf3_p2 CCCATTCCTCGAAAGACGTT AGCTATAGCCCTTACCCCTG 50.0 55.0 57.9 58.0 932-938 bp 1.0 82.2
psaA-ycf3_p3 AGAAATGACCGGGTTTAGCC AGCCCTTACCCCTGGTAATT 50.0 50.0 57.9 58.0 944-950 bp 1.0 82.2
psaA-ycf3_p4 CCCATTCCTCGAAAGACGTT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 957-963 bp 1.0 82.1
psaA-ycf3_p5 AGAAATGACCGGGTTTAGCC AGCTATAGCCCTTACCCCTG 50.0 55.0 57.9 58.0 950-956 bp 1.0 81.9