Hotspot

psbC-trnS-UGA

LSC · 39144-39384 · 241 bp

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Pi 0.0581
MarkerSeek score 78.00
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
241
variable_sites
14
pi
0.0581
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 AGCTGCAGCAGGATTTGAAA GAGAGATGGCTGAGTGGTTG 45.0 55.0 58.4 58.0 406 bp 1.0 79.6
psbC-trnS-UGA_p2 AGCTGCAGCAGGATTTGAAA GCTGAGTGGTTGATAGCTCC 45.0 55.0 58.4 58.1 398 bp 1.0 79.4
psbC-trnS-UGA_p3 AGCTGCAGCAGGATTTGAAA GGCTGAGTGGTTGATAGCTC 45.0 55.0 58.4 58.1 399 bp 1.0 79.4
psbC-trnS-UGA_p4 AGCTGCAGCAGGATTTGAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.4 58.1 391 bp 1.0 79.4
psbC-trnS-UGA_p5 TAGGTCATTTATGGCACGCG GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.4 58.0 440 bp 1.0 79.0