Hotspot

trnS-GCU-trnG-UCC

LSC · 9454-10293 · 840 bp

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Pi 0.0225
MarkerSeek score 68.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
840
variable_sites
18
pi
0.0225
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 952-1001 bp 1.0 79.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 951-1000 bp 1.0 78.9
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.2 57.3 1006-1055 bp 1.0 77.5
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 920-969 bp 1.0 76.6
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 919-968 bp 1.0 76.6