Hotspot

trnQ-UUG-psbK

LSC · 7826-8558 · 733 bp

Back to Cassia

Pi 0.0237
MarkerSeek score 64.40
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
733
variable_sites
16
pi
0.0237
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GATATGCTCTGGGACGGAAG ACAGACCAAACTAAAGATATTAAGCA 55.0 30.8 57.6 56.9 800-840 bp 1.0 45.6
trnQ-UUG-psbK_p2 GACCAAAACCCGTTGCCTTA ACAGACCAAACTAAAGATATTAAGCA 50.0 30.8 58.7 56.9 758-798 bp 1.0 45.6
trnQ-UUG-psbK_p3 GACCAAAACCCGTTGCCTTA ACAAATACAGACCAAACTAAAGA 50.0 30.4 58.7 53.9 764-804 bp 1.0 45.6
trnQ-UUG-psbK_p4 AATGGATATGCTCTGGGACG ACAGACCAAACTAAAGATATTAAGCA 50.0 30.8 57.2 56.9 804-844 bp 1.0 45.6
trnQ-UUG-psbK_p5 GATATGCTCTGGGACGGAAG ACAAATACAGACCAAACTAAAGA 55.0 30.4 57.6 53.9 806-846 bp 1.0 45.5