Hotspot

rps16-trnQ-UUG

LSC · 6686-7753 · 1068 bp

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Pi 0.0549
MarkerSeek score 79.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1068
variable_sites
39
pi
0.0549
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.66
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 927-1148 bp 1.0 86.4
rps16-trnQ-UUG_p2 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 929-1150 bp 1.0 85.3
rps16-trnQ-UUG_p3 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 915-1136 bp 1.0 84.9
rps16-trnQ-UUG_p4 CATGTCTTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 930-1151 bp 1.0 84.7
rps16-trnQ-UUG_p5 TGTCTTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 928-1149 bp 1.0 83.6