Hotspot

infA

LSC · 81591-81881 · 291 bp

Back to Casearia

Pi 0.0246

MarkerSeek score 65.00

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 291
variable_sites: 25
pi: 0.0246
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	ATTATAATTCGTCCCCGCCG	GAAGGAATCGGCGGAGAAAT	50.0	50.0	57.9	58.0	508-603 bp	1.0	90.9
infA_p2	CAGCAAATGGTGTCCCTCTT	GAAGGAATCGGCGGAGAAAT	50.0	50.0	58.1	58.0	884-985 bp	1.0	89.6
infA_p3	ATTATAATTCGTCCCCGCCG	GAAGGAATAGGAAAGGGCCG	50.0	55.0	57.9	58.0	751-773 bp	0.5	65.6
infA_p4	CAGCAAATGGTGTCCCTCTT	GAAGGAATAGGAAAGGGCCG	50.0	55.0	58.1	58.0	1127-1155 bp	0.5	64.4
infA_p5	GCTCTTAATGCCGCATCTCT	GAAGGAATAGGAAAGGGCCG	50.0	55.0	58.1	58.0	1236-1264 bp	0.5	63.8