Hotspot

rpl22-rps19

LSC · 86078-86251 · 174 bp

Back to Casearia

Pi 0.064

MarkerSeek score 77.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 31
pi: 0.064
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GGAACGTCCACGAATCTGAT	TTTCCGGGGACATGCAAAAA	50.0	45.0	57.7	58.6	278-352 bp	1.0	85.0
rpl22-rps19_p2	CGTAGGAACGTCCACGAATC	TTTCCGGGGACATGCAAAAA	55.0	45.0	58.5	58.6	282-356 bp	1.0	83.7
rpl22-rps19_p3	TGTTGACCTAAAGCGGATGC	TTTCCGGGGACATGCAAAAA	50.0	45.0	58.6	58.6	220-294 bp	1.0	82.9
rpl22-rps19_p4	AGGAACGTCCACGAATCTGA	TTTCCGGGGACATGCAAAAA	50.0	45.0	58.7	58.6	279-353 bp	1.0	81.3
rpl22-rps19_p5	GGAACGTCCACGAATCTGAT	TTCCGGGGACATGCAAAAA	50.0	47.4	57.7	57.9	277-351 bp	1.0	80.6