Hotspot

psaJ-rpl33

LSC · 68424-68914 · 491 bp

Back to Casearia

Pi 0.0313

MarkerSeek score 73.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 45
pi: 0.0313
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGGTCTTTAGCGGGTCTTTT	CATCTTTACCATTGGCCAT	50.0	42.1	57.8	52.9	553-599 bp	1.0	50.6
psaJ-rpl33_p2	GGTCTTTAGCGGGTCTTTTGA	CATCTTTACCATTGGCCAT	47.6	42.1	58.2	52.9	552-598 bp	1.0	50.6
psaJ-rpl33_p3	GGTCTTTAGCGGGTCTTTTG	CATCTTTACCATTGGCCAT	50.0	42.1	56.7	52.9	552-598 bp	1.0	50.6
psaJ-rpl33_p4	TATGGTTCGGGTCTTTAGCG	CATCTTTACCATTGGCCAT	50.0	42.1	57.4	52.9	561-607 bp	1.0	50.5
psaJ-rpl33_p5	ATGGTTCGGGTCTTTAGCG	CATCTTTACCATTGGCCAT	52.6	42.1	57.5	52.9	560-606 bp	1.0	50.5