Hotspot

rbcL-accD

LSC · 57473-58251 · 779 bp

Back to Casearia

Pi 0.0385

MarkerSeek score 79.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 83
pi: 0.0385
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	AGTGTCATCCGCGCTTAAAT	55.0	45.0	58.0	58.0	1066-1109 bp	1.0	90.9
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	AGTGTCATCCGCGCTTAAAT	50.0	45.0	58.0	58.0	1111-1154 bp	1.0	90.7
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	AAATCGCTACCATTCGTGCT	55.0	45.0	58.0	58.0	1455-1507 bp	0.9	85.3
rbcL-accD_p4	AATGAGGGACGTGATCTTGC	AAATCGCTACCATTCGTGCT	50.0	45.0	58.0	58.0	1500-1552 bp	0.9	85.2
rbcL-accD_p5	CGGAGGAACTTTAGGGCATC	AGTGTCATCCGCGCTTAAAT	55.0	45.0	58.0	58.0	1239 bp	0.1	43.4