Hotspot

ndhC-trnV-UAC

LSC · 50792-52196 · 1405 bp

Back to Casearia

Pi 0.0353

MarkerSeek score 74.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1405
variable_sites: 140
pi: 0.0353
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGATAAAAGGCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1087-1536 bp	1.0	89.8
ndhC-trnV-UAC_p2	AATTGGGGATAAAAGGCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1091-1540 bp	1.0	88.7
ndhC-trnV-UAC_p3	GGGGATAAAAGGCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1086-1535 bp	1.0	87.0
ndhC-trnV-UAC_p4	AATTGGGGATAAAAGGCCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.2	57.5	1090-1539 bp	1.0	86.0
ndhC-trnV-UAC_p5	GGGGATAAAAGGCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1075-1524 bp	1.0	85.9