Hotspot

psbZ-trnG-GCC

LSC · 36251-36759 · 509 bp

Back to Casearia

Pi 0.0488

MarkerSeek score 78.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 509
variable_sites: 72
pi: 0.0488
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGTTGGTCAAGCAACAAAA	TACCATTCGACTATATCCGC	45.0	45.0	57.7	54.3	574-641 bp	1.0	59.9
psbZ-trnG-GCC_p2	CGGTTGGTCAAGCAACAAAA	TTACCATTCGACTATATCCGC	45.0	42.9	57.7	55.1	575-642 bp	1.0	58.6
psbZ-trnG-GCC_p3	TTTCTTCTCCTGACGGTTGG	TACCATTCGACTATATCCGC	50.0	45.0	57.5	54.3	587-654 bp	1.0	57.8
psbZ-trnG-GCC_p4	TTCTTCTCCTGACGGTTGGT	TACCATTCGACTATATCCGC	50.0	45.0	58.6	54.3	586-653 bp	1.0	57.6
psbZ-trnG-GCC_p5	CGGTTGGTCAAGCAACAAAA	TTTACCATTCGACTATATCCGC	45.0	40.9	57.7	55.9	576-643 bp	1.0	56.8