Hotspot

trnT-GGU-psbD

LSC · 31344-32945 · 1602 bp

Back to Casearia

Pi 0.0244

MarkerSeek score 80.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1602
variable_sites: 133
pi: 0.0244
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CGGACCAACCTACAAAAACG	55.0	50.0	58.1	57.3	1554-1827 bp	1.0	84.3
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GCCGGACCAACCTACAAAAA	55.0	50.0	58.1	58.7	1556-1829 bp	1.0	84.2
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	CGGACCAACCTACAAAAACG	50.0	50.0	58.1	57.3	1557-1830 bp	1.0	84.1
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GCCGGACCAACCTACAAAAA	50.0	50.0	58.1	58.7	1559-1832 bp	1.0	84.1
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GAAAGAGCAATAGGCCGGAC	55.0	55.0	58.1	58.7	1569-1842 bp	1.0	84.0