Hotspot

trnS-GCU-trnG-UCC

LSC · 8271-8618 · 348 bp

Back to Casearia

Pi 0.0529
MarkerSeek score 91.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
348
variable_sites
57
pi
0.0529
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.94
markerseek_score
91.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ATGCACCGAGAGATCCCTTA 50.0 50.0 58.0 57.9 430-521 bp 0.9 87.3
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA TATGCACCGAGAGATCCCTT 50.0 50.0 58.0 57.9 431-522 bp 0.9 87.3
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC ATGCACCGAGAGATCCCTTA 50.0 50.0 58.0 57.9 429-520 bp 0.9 87.2
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TATGCACCGAGAGATCCCTT 50.0 50.0 58.0 57.9 430-521 bp 0.9 87.2
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA TGCACCGAGAGATCCCTTAA 50.0 50.0 58.0 58.1 429-520 bp 0.9 86.8