Hotspot

trnK-UUU-trnQ-UUG

LSC · 4247-6880 · 2634 bp

Back to Casearia

Pi 0.0332
MarkerSeek score 73.70
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2634
variable_sites
256
pi
0.0332
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 TGTAAAAGCTGTTAGCCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1275-2744 bp 1.0 90.5
trnK-UUU-trnQ-UUG_p2 AAAGCTGTTAGCCGCACTTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1271-2740 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p3 AAGCTGTTAGCCGCACTTAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1270-2739 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p4 AGCTGTTAGCCGCACTTAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1269-2738 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p5 TAAAAGCTGTTAGCCGCACT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1273-2742 bp 1.0 90.0