Hotspot

psbA-trnK-UUU

LSC · 1341-1607 · 267 bp

Back to Casearia

Pi 0.0318

MarkerSeek score 73.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 32
pi: 0.0318
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAAAGACGGTTTTCGGTGCT	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.8	58.4	450-497 bp	1.0	82.3
psbA-trnK-UUU_p2	GTAAAGACGGTTTTCGGTGC	AAAATGCAAGCACGGTTTGG	50.0	45.0	57.7	58.4	451-498 bp	1.0	81.8
psbA-trnK-UUU_p3	TAAAGACGGTTTTCGGTGCT	TGAAAAATGCAAGCACGGTT	45.0	40.0	57.8	57.4	453-500 bp	1.0	80.9
psbA-trnK-UUU_p4	TAAAGACGGTTTTCGGTGCT	AAAGCCGTGTGCAATGAAAA	45.0	40.0	57.8	57.4	467-514 bp	1.0	80.7
psbA-trnK-UUU_p5	TAAAGACGGTTTTCGGTGCT	AAGCCGTGTGCAATGAAAAA	45.0	40.0	57.8	57.4	466-513 bp	1.0	80.7