Hotspot

ycf1

SSC · 128299-133893 · 5595 bp

Back to Caryota

Pi 0.00232

MarkerSeek score 53.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5595
variable_sites: 13
pi: 0.00232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCTTTTCTTGATTCATTCCCA	TCGACATTGAGACCTGGACT	36.4	50.0	55.9	58.1	187 bp	1.0	47.6
ycf1_p2	AACCTTTTCTTGATTCATTCCCA	TCGACATTGAGACCTGGACT	34.8	50.0	56.6	58.1	188 bp	1.0	45.4
ycf1_p3	ACCTTTTCTTGATTCATTCCCATC	TCGACATTGAGACCTGGACT	37.5	50.0	57.5	58.1	187 bp	1.0	44.4
ycf1_p4	ACCTTTTCTTGATTCATTCCCAT	TCGACATTGAGACCTGGACT	34.8	50.0	56.4	58.1	187 bp	1.0	43.8
ycf1_p5	AAACCTTTTCTTGATTCATTCCCA	TCGACATTGAGACCTGGACT	33.3	50.0	57.3	58.1	189 bp	1.0	42.7