Hotspot

ccsA

SSC · 118539-119507 · 969 bp

Back to Caryota

Pi 0.00206

MarkerSeek score 50.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTGGCGGCATCCTATAAA	ACTCGAACAAGCAAACTTTCG	50.0	42.9	57.5	57.7	1121 bp	1.0	65.2
ccsA_p2	GAGTGGCGGCATCCTATAAA	CTCGAACAAGCAAACTTTCGT	50.0	42.9	57.5	57.7	1120 bp	1.0	65.2
ccsA_p3	GAGTGGCGGCATCCTATAAA	TCGAACAAGCAAACTTTCGT	50.0	40.0	57.5	56.5	1119 bp	1.0	64.0
ccsA_p4	GAGTGGCGGCATCCTATAAA	TCGAACAAGCAAACTTTCGTT	50.0	38.1	57.5	57.2	1119 bp	1.0	61.8
ccsA_p5	AGTGGCGGCATCCTATAAAA	CTCGAACAAGCAAACTTTCGT	45.0	42.9	57.0	57.7	1119 bp	1.0	61.4