Hotspot

rpl32-trnL-UAG

SSC · 117587-118382 · 796 bp

Back to Caryota

Pi 0.00378

MarkerSeek score 60.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 796
variable_sites: 3
pi: 0.00378
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTCTACCGGACATTCAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	55.4	58.0	887-889 bp	1.0	59.4
rpl32-trnL-UAG_p2	GGTTTCTACCGGACATTCAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	55.4	58.0	888-890 bp	1.0	59.4
rpl32-trnL-UAG_p3	GGTTTCTACCGGACATTCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	55.4	57.9	897-899 bp	1.0	58.5
rpl32-trnL-UAG_p4	GGTTTCTACCGGACATTCAA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	55.4	57.8	896-898 bp	1.0	57.9
rpl32-trnL-UAG_p5	GGTTTCTACCGGACATTCAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	55.4	57.8	895-897 bp	1.0	57.9