Hotspot

ndhF-rpl32

SSC · 117035-117412 · 378 bp

Back to Caryota

Pi 0.00548
MarkerSeek score 60.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
378
variable_sites
2
pi
0.00548
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAATAAGT ACCGATTTCGCTAAAGAAAAAGA 40.0 34.8 55.3 57.1 591-604 bp 1.0 42.0
ndhF-rpl32_p2 TTGTTGCTGTCGGAATAAGT GAAACCGATTTCGCTAAAGA 40.0 40.0 55.3 54.1 594-607 bp 1.0 42.0
ndhF-rpl32_p3 TTGTTGCTGTCGGAATAAGT GAAACCGATTTCGCTAAAGAA 40.0 38.1 55.3 54.9 594-607 bp 1.0 42.0
ndhF-rpl32_p4 TTTGTTGCTGTCGGAATAAGT ACCGATTTCGCTAAAGAAAAAGA 38.1 34.8 56.1 57.1 592-605 bp 1.0 42.0
ndhF-rpl32_p5 TTTGTTGCTGTCGGAATAAGT GAAACCGATTTCGCTAAAGA 38.1 40.0 56.1 54.1 595-608 bp 1.0 42.0