Hotspot

accD-psaI

LSC · 61843-62900 · 1058 bp

Back to Caryota

Pi 0.00284

MarkerSeek score 60.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 3
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1172-1250 bp	1.0	78.3
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1171-1249 bp	1.0	78.3
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1178-1256 bp	1.0	77.7
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1177-1255 bp	1.0	77.7
accD-psaI_p5	TTGACCCAATTGTACCACGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	1226-1304 bp	1.0	74.9