primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	GGGTATTGCTCCATTCCTTCA	GTGTGCTTGGGAGTTCTTGA	21	20	47.6	50.0	57.7	58.0	0.0	0.0	1.329816	92	1153	1156-1156	1.000000	69.4
psbA_p2	psbA	2	TGGGTATTGCTCCATTCCTTC	GTGTGCTTGGGAGTTCTTGA	21	20	47.6	50.0	57.7	58.0	0.0	0.0	1.329816	92	1153	1157-1157	1.000000	69.4
psbA_p3	psbA	3	GGGTATTGCTCCATTCCTTCA	ATACGTGTGCTTGGGAGTTC	21	20	47.6	50.0	57.7	57.9	0.0	0.0	1.391897	92	1153	1160-1160	1.000000	68.9
psbA_p4	psbA	4	TGGGTATTGCTCCATTCCTTC	ATACGTGTGCTTGGGAGTTC	21	20	47.6	50.0	57.7	57.9	0.0	0.0	1.391897	92	1153	1161-1161	1.000000	68.9
psbA_p5	psbA	5	TGGGTATTGCTCCATTCCTT	GTGTGCTTGGGAGTTCTTGA	20	20	45.0	50.0	56.5	58.0	0.0	0.0	1.555803	92	1153	1157-1157	1.000000	67.6
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCACGATCATGAGCAAATGTG	AACGGTAGAGTACTCGGCTT	21	20	47.6	50.0	57.9	58.2	12.1	17.1	1.229398	3227	4062	964-967	1.000000	70.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCACGATCATGAGCAAATGTG	ACGGTAGAGTACTCGGCTTT	21	20	47.6	50.0	57.9	58.2	12.1	17.1	1.229398	3227	4062	963-966	1.000000	70.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCACGATCATGAGCAAATGTG	GTCGTGCAGTGCAATTCAAT	21	20	47.6	45.0	57.9	57.7	12.1	21.7	1.397533	3227	4062	1059-1062	1.000000	69.3
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCACGATCATGAGCAAATGT	AACGGTAGAGTACTCGGCTT	21	20	42.9	50.0	58.3	58.2	12.1	17.1	1.453374	3227	4062	965-968	1.000000	68.9
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACCACGATCATGAGCAAATGT	ACGGTAGAGTACTCGGCTTT	21	20	42.9	50.0	58.3	58.2	12.1	17.1	1.453374	3227	4062	964-967	1.000000	68.9
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	AACGGAAAGACCCCTTAACT	20	20	45.0	45.0	58.0	56.4	0.0	0.0	1.674622	8418	9947	1550-1642	1.000000	70.7
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AGAAGAAGCGGAGAGAGAGG	AACGGAAAGACCCCTTAACT	20	20	55.0	45.0	58.2	56.4	0.0	0.0	1.873708	8418	9947	1606-1698	1.000000	69.1
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ATTAGCAATCCGCCGCTTTA	AAACGGAAAGACCCCTTAACT	20	21	45.0	42.9	58.0	57.1	0.0	0.0	1.908745	8418	9947	1551-1643	1.000000	68.8
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	CGGAGAGAGAGGGATTCGAA	AACGGAAAGACCCCTTAACT	20	20	55.0	45.0	58.3	56.4	0.0	0.0	1.954878	8418	9947	1598-1690	1.000000	68.4
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	GCTTTAGTCCACTCAGCCAT	AACGGAAAGACCCCTTAACT	20	20	50.0	45.0	57.6	56.4	0.9	0.0	2.046364	8418	9947	1536-1628	1.000000	67.7
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	20	20	45.0	45.0	58.0	58.6	1.4	16.5	0.624009	14843	15723	952-970	1.000000	76.5
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	20	20	45.0	50.0	58.0	57.4	1.4	21.3	0.625447	14843	15723	924-942	1.000000	76.5
atpH-atpI_p3	atpH-atpI	3	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	58.0	58.6	3.3	16.5	0.633001	14843	15723	1035-1053	1.000000	76.3
atpH-atpI_p4	atpH-atpI	4	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	58.0	57.4	3.3	21.3	0.634438	14843	15723	1007-1025	1.000000	76.3
atpH-atpI_p5	atpH-atpI	5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	57.9	58.6	0.0	16.5	0.697901	14843	15723	1008-1026	1.000000	75.8
petN-psbM_p1	petN-psbM	1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.592428	30494	31361	1012-1024	1.000000	70.9
petN-psbM_p2	petN-psbM	2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.716560	30494	31361	1007-1019	1.000000	69.9
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	30494	31361	957-969	1.000000	69.8
petN-psbM_p4	petN-psbM	4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.6	57.6	0.0	0.0	1.963622	30494	31361	1016-1028	1.000000	67.9
petN-psbM_p5	petN-psbM	5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.6	57.6	0.0	0.0	2.055340	30494	31361	1015-1027	1.000000	67.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48145	49281	1097-1187	1.000000	83.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	48145	49281	1118-1208	1.000000	82.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48145	49281	1105-1195	1.000000	82.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48145	49281	1129-1219	1.000000	82.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.248324	48145	49281	1150-1240	1.000000	82.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	50303	51018	826-826	1.000000	78.7
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	50303	51018	816-816	1.000000	78.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300111	50303	51018	872-872	1.000000	77.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	50303	51018	869-869	1.000000	77.5
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	50303	51018	868-868	1.000000	77.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	8.5	0.0	0.269796	52710	54358	1791-1793	1.000000	78.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	8.5	0.0	0.280450	52710	54358	1783-1785	1.000000	78.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	58.1	58.5	8.5	0.0	0.546572	52710	54358	1873-1875	1.000000	76.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.9	58.5	8.5	0.0	0.557226	52710	54358	1865-1867	1.000000	76.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	8.5	0.0	0.613568	52710	54358	1790-1792	1.000000	75.9
accD-psaI_p1	accD-psaI	1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	12.6	26.9	0.249230	61843	62900	1172-1250	1.000000	78.3
accD-psaI_p2	accD-psaI	2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	12.6	26.9	0.249230	61843	62900	1171-1249	1.000000	78.3
accD-psaI_p3	accD-psaI	3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.8	57.9	12.6	26.8	0.325764	61843	62900	1178-1256	1.000000	77.7
accD-psaI_p4	accD-psaI	4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.8	57.9	12.6	26.8	0.325764	61843	62900	1177-1255	1.000000	77.7
accD-psaI_p5	accD-psaI	5	TTGACCCAATTGTACCACGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.4	58.0	4.4	26.9	0.669158	61843	62900	1226-1304	1.000000	74.9
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.350824	66003	67032	1182-1182	1.000000	77.3
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.466931	66003	67032	1178-1178	1.000000	76.4
petA-psbJ_p3	petA-psbJ	3	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.468635	66003	67032	1181-1181	1.000000	76.4
petA-psbJ_p4	petA-psbJ	4	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.2	58.3	13.8	0.0	0.491221	66003	67032	1179-1179	1.000000	76.2
petA-psbJ_p5	petA-psbJ	5	CATTACGTGTCCAAGGCCTT	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.2	57.6	13.8	0.0	0.607328	66003	67032	1175-1175	1.000000	75.3
rpl33-rps18_p1	rpl33-rps18	1	CAAGCATACGATTCATGGGGA	TAATCGATTCGATCCCCCGA	21	20	47.6	50.0	58.2	58.1	0.0	21.0	1.236280	71186	71483	418-421	1.000000	71.6
rpl33-rps18_p2	rpl33-rps18	2	AAGCATACGATTCATGGGGA	TAATCGATTCGATCCCCCGA	20	20	45.0	50.0	56.7	58.1	0.0	21.0	1.409084	71186	71483	417-420	1.000000	70.3
rpl33-rps18_p3	rpl33-rps18	3	AGCATACGATTCATGGGGAA	TAATCGATTCGATCCCCCGA	20	20	45.0	50.0	56.7	58.1	0.0	21.0	1.409084	71186	71483	416-419	1.000000	70.3
rpl33-rps18_p4	rpl33-rps18	4	CAAGCATACGATTCATGGGG	TAATCGATTCGATCCCCCGA	20	20	50.0	50.0	56.6	58.1	0.0	21.0	1.444160	71186	71483	418-421	1.000000	70.0
rpl33-rps18_p5	rpl33-rps18	5	ACAAGCATACGATTCATGGGG	TAATCGATTCGATCCCCCGA	21	20	47.6	50.0	58.4	58.1	0.0	21.0	1.510691	71186	71483	419-422	1.000000	69.4
ndhF-rpl32_p1	ndhF-rpl32	1	TTGTTGCTGTCGGAATAAGT	ACCGATTTCGCTAAAGAAAAAGA	20	23	40.0	34.8	55.3	57.1	0.0	0.0	6.637751	117035	117412	591-604	1.000000	42.0
ndhF-rpl32_p2	ndhF-rpl32	2	TTGTTGCTGTCGGAATAAGT	GAAACCGATTTCGCTAAAGA	20	20	40.0	40.0	55.3	54.1	0.0	0.0	6.637899	117035	117412	594-607	1.000000	42.0
ndhF-rpl32_p3	ndhF-rpl32	3	TTGTTGCTGTCGGAATAAGT	GAAACCGATTTCGCTAAAGAA	20	21	40.0	38.1	55.3	54.9	0.0	0.0	6.797111	117035	117412	594-607	1.000000	42.0
ndhF-rpl32_p4	ndhF-rpl32	4	TTTGTTGCTGTCGGAATAAGT	ACCGATTTCGCTAAAGAAAAAGA	21	23	38.1	34.8	56.1	57.1	0.0	0.0	6.837542	117035	117412	592-605	1.000000	42.0
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCTGTCGGAATAAGT	GAAACCGATTTCGCTAAAGA	21	20	38.1	40.0	56.1	54.1	0.0	0.0	6.837690	117035	117412	595-608	1.000000	42.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTTTCTACCGGACATTCAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	55.4	58.0	9.5	0.0	2.630573	117587	118382	887-889	1.000000	59.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGTTTCTACCGGACATTCAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	55.4	58.0	9.5	0.0	2.631944	117587	118382	888-890	1.000000	59.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTTTCTACCGGACATTCAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	55.4	57.9	9.5	0.0	2.747020	117587	118382	897-899	1.000000	58.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGTTTCTACCGGACATTCAA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	55.4	57.8	9.5	0.0	2.819038	117587	118382	896-898	1.000000	57.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGTTTCTACCGGACATTCAA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	55.4	57.8	9.5	0.0	2.819038	117587	118382	895-897	1.000000	57.9
ccsA_p1	ccsA	1	GAGTGGCGGCATCCTATAAA	ACTCGAACAAGCAAACTTTCG	20	21	50.0	42.9	57.5	57.7	17.2	0.0	1.863015	118539	119507	1121-1121	1.000000	65.2
ccsA_p2	ccsA	2	GAGTGGCGGCATCCTATAAA	CTCGAACAAGCAAACTTTCGT	20	21	50.0	42.9	57.5	57.7	17.2	0.0	1.863015	118539	119507	1120-1120	1.000000	65.2
ccsA_p3	ccsA	3	GAGTGGCGGCATCCTATAAA	TCGAACAAGCAAACTTTCGT	20	20	50.0	40.0	57.5	56.5	17.2	0.0	2.022987	118539	119507	1119-1119	1.000000	64.0
ccsA_p4	ccsA	4	GAGTGGCGGCATCCTATAAA	TCGAACAAGCAAACTTTCGTT	20	21	50.0	38.1	57.5	57.2	17.2	0.0	2.294416	118539	119507	1119-1119	1.000000	61.8
ccsA_p5	ccsA	5	AGTGGCGGCATCCTATAAAA	CTCGAACAAGCAAACTTTCGT	20	21	45.0	42.9	57.0	57.7	17.2	0.0	2.340463	118539	119507	1119-1119	1.000000	61.4
ccsA-ndhD_p1	ccsA-ndhD	1	ACGAATTCGGCACTTATAGCT	TCAGTTGACAGGGTACAAGC	21	20	42.9	50.0	57.5	57.5	29.8	0.0	1.998149	119508	119724	385-385	1.000000	65.1
ccsA-ndhD_p2	ccsA-ndhD	2	ACGAATTCGGCACTTATAGCT	CAGTTGACAGGGTACAAGCT	21	20	42.9	50.0	57.5	57.4	29.8	0.0	2.005202	119508	119724	384-384	1.000000	65.0
ccsA-ndhD_p3	ccsA-ndhD	3	ACGAATTCGGCACTTATAGC	TCAGTTGACAGGGTACAAGC	20	20	45.0	50.0	56.0	57.5	29.8	0.0	2.509324	119508	119724	385-385	1.000000	61.0
ccsA-ndhD_p4	ccsA-ndhD	4	ACGAATTCGGCACTTATAGCT	ATCAGTTGACAGGGTACAAGC	21	21	42.9	47.6	57.5	57.9	29.8	0.0	2.513723	119508	119724	386-386	1.000000	60.9
ccsA-ndhD_p5	ccsA-ndhD	5	ACGAATTCGGCACTTATAGC	CAGTTGACAGGGTACAAGCT	20	20	45.0	50.0	56.0	57.4	29.8	0.0	2.516376	119508	119724	384-384	1.000000	60.9
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	121595	122233	794-833	1.000000	79.1
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	121595	122233	793-832	1.000000	79.1
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	121595	122233	814-853	1.000000	79.0
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	121595	122233	792-831	1.000000	77.9
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	121595	122233	769-808	1.000000	77.6
ycf1_p1	ycf1	1	ACCTTTTCTTGATTCATTCCCA	TCGACATTGAGACCTGGACT	22	20	36.4	50.0	55.9	58.1	0.0	0.0	4.209932	129452	129571	187-187	1.000000	47.6
ycf1_p2	ycf1	2	AACCTTTTCTTGATTCATTCCCA	TCGACATTGAGACCTGGACT	23	20	34.8	50.0	56.6	58.1	0.0	0.0	4.484950	129452	129571	188-188	1.000000	45.4
ycf1_p3	ycf1	3	ACCTTTTCTTGATTCATTCCCATC	TCGACATTGAGACCTGGACT	24	20	37.5	50.0	57.5	58.1	0.0	0.0	4.611232	129452	129571	187-187	1.000000	44.4
ycf1_p4	ycf1	4	ACCTTTTCTTGATTCATTCCCAT	TCGACATTGAGACCTGGACT	23	20	34.8	50.0	56.4	58.1	0.0	0.0	4.690484	129452	129571	187-187	1.000000	43.8
ycf1_p5	ycf1	5	AAACCTTTTCTTGATTCATTCCCA	TCGACATTGAGACCTGGACT	24	20	33.3	50.0	57.3	58.1	0.0	0.0	4.821063	129452	129571	189-189	1.000000	42.7